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quickblock western blocking solution p0252  (Beyotime)


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    Structured Review

    Beyotime quickblock western blocking solution p0252
    Quickblock Western Blocking Solution P0252, supplied by Beyotime, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/product/quickblock+western+blocking+solution+p0252/quickblocktm+blocking+buffer+for+western+blot/pmc11349537-193-7-12
    Average 90 stars, based on 1 article reviews
    quickblock western blocking solution p0252 - by Bioz Stars, 2026-09
    90/100 stars

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    Related Articles

    Blocking Assay:

    Article Title: Lactic acid promotes nucleus pulposus cell senescence and corresponding intervertebral disc degeneration via interacting with Akt.
    Article Snippet: 1 Department of Orthopedics, Xinqiao Hospital, Army Military Medical University, Chongqing 400037, China 2 Institute of Basic Theory of Traditional Chinese Medicine, China Academy of Chinese Medical Science, Beijing 100000, China 3 State Key Laboratory of Trauma, Burn and Combined Injury, Army Military Medical University, Chongqing 400038, China 4 Department of Orthopedics, General Hospital of Central Theater Command of PLA, Wuhan 430000, China Abstract The accumulation of metabolites in the intervertebral disc is considered an important cause of intervertebral disc degeneration (IVDD).. Lactic acid, which is a metabolite that is produced by cellular anaerobic glycolysis, has been proven to be closely associated with IVDD.. However, little is known about the role of lactic acid in nucleus pulposus cells (NPCs) senescence and oxidative stress.

    Article Title: Dynamics of N6 ‐methyladenosine modification during aging and their potential roles in the degeneration of intervertebral disc
    Article Snippet: Equal amounts of total protein (20 μg) of each sample were separated on 5%–12% gradient SDS‐PAGE gel (P0012AC, Beyotime) and transferred to a 0.45‐um PVDF membrane (Merck, Germany). .. After the membrane was blocked by QuickBlock Western blocking solution (P0252, Beyotime, China) for 1 h, and subsequently incubated with different kinds of primary antibodies (Table ) at 4°C overnight with rotation (the housekeeping proteins used in this study were GAPDH). ..

    Article Title: Gli1 depletion induces oxidative stress and apoptosis of nucleus pulposus cells via Fos in intervertebral disc degeneration.
    Article Snippet: The protein samples were separated using SDS-PAGE and transferred to a 0.45-um PVDF membrane (Merck, Germany). .. Membranes were blocked by QuickBlock Western blocking solution (P0252, Beyotime, China) for 1 h and incubated with different kinds of primary antibodies (Table 1) at 4 C overnight with rotation (the housekeeping proteins used in this study were GAPDH (whole protein/cytoplasmic protein) and Lamin B1 (nuclear protein)). ..

    Article Title: Gli1 depletion induces oxidative stress and apoptosis of nucleus pulposus cells via Fos in intervertebral disc degeneration
    Article Snippet: The protein samples were separated using SDS-PAGE and transferred to a 0.45-um PVDF membrane (Merck, Germany). .. Membranes were blocked by QuickBlock Western blocking solution (P0252, Beyotime, China) for 1 h and incubated with different kinds of primary antibodies ( ) at 4 °C overnight with rotation (the housekeeping proteins used in this study were GAPDH (whole protein/cytoplasmic protein) and Lamin B1 (nuclear protein)). ..

    Western Blot:

    Article Title: Lactic acid promotes nucleus pulposus cell senescence and corresponding intervertebral disc degeneration via interacting with Akt.
    Article Snippet: 1 Department of Orthopedics, Xinqiao Hospital, Army Military Medical University, Chongqing 400037, China 2 Institute of Basic Theory of Traditional Chinese Medicine, China Academy of Chinese Medical Science, Beijing 100000, China 3 State Key Laboratory of Trauma, Burn and Combined Injury, Army Military Medical University, Chongqing 400038, China 4 Department of Orthopedics, General Hospital of Central Theater Command of PLA, Wuhan 430000, China Abstract The accumulation of metabolites in the intervertebral disc is considered an important cause of intervertebral disc degeneration (IVDD).. Lactic acid, which is a metabolite that is produced by cellular anaerobic glycolysis, has been proven to be closely associated with IVDD.. However, little is known about the role of lactic acid in nucleus pulposus cells (NPCs) senescence and oxidative stress.

    Article Title: Dynamics of N6 ‐methyladenosine modification during aging and their potential roles in the degeneration of intervertebral disc
    Article Snippet: Equal amounts of total protein (20 μg) of each sample were separated on 5%–12% gradient SDS‐PAGE gel (P0012AC, Beyotime) and transferred to a 0.45‐um PVDF membrane (Merck, Germany). .. After the membrane was blocked by QuickBlock Western blocking solution (P0252, Beyotime, China) for 1 h, and subsequently incubated with different kinds of primary antibodies (Table ) at 4°C overnight with rotation (the housekeeping proteins used in this study were GAPDH). ..

    Article Title: Gli1 depletion induces oxidative stress and apoptosis of nucleus pulposus cells via Fos in intervertebral disc degeneration.
    Article Snippet: The protein samples were separated using SDS-PAGE and transferred to a 0.45-um PVDF membrane (Merck, Germany). .. Membranes were blocked by QuickBlock Western blocking solution (P0252, Beyotime, China) for 1 h and incubated with different kinds of primary antibodies (Table 1) at 4 C overnight with rotation (the housekeeping proteins used in this study were GAPDH (whole protein/cytoplasmic protein) and Lamin B1 (nuclear protein)). ..

    Article Title: Gli1 depletion induces oxidative stress and apoptosis of nucleus pulposus cells via Fos in intervertebral disc degeneration
    Article Snippet: The protein samples were separated using SDS-PAGE and transferred to a 0.45-um PVDF membrane (Merck, Germany). .. Membranes were blocked by QuickBlock Western blocking solution (P0252, Beyotime, China) for 1 h and incubated with different kinds of primary antibodies ( ) at 4 °C overnight with rotation (the housekeeping proteins used in this study were GAPDH (whole protein/cytoplasmic protein) and Lamin B1 (nuclear protein)). ..

    Incubation:

    Article Title: Lactic acid promotes nucleus pulposus cell senescence and corresponding intervertebral disc degeneration via interacting with Akt.
    Article Snippet: 1 Department of Orthopedics, Xinqiao Hospital, Army Military Medical University, Chongqing 400037, China 2 Institute of Basic Theory of Traditional Chinese Medicine, China Academy of Chinese Medical Science, Beijing 100000, China 3 State Key Laboratory of Trauma, Burn and Combined Injury, Army Military Medical University, Chongqing 400038, China 4 Department of Orthopedics, General Hospital of Central Theater Command of PLA, Wuhan 430000, China Abstract The accumulation of metabolites in the intervertebral disc is considered an important cause of intervertebral disc degeneration (IVDD).. Lactic acid, which is a metabolite that is produced by cellular anaerobic glycolysis, has been proven to be closely associated with IVDD.. However, little is known about the role of lactic acid in nucleus pulposus cells (NPCs) senescence and oxidative stress.

    Article Title: Dynamics of N6 ‐methyladenosine modification during aging and their potential roles in the degeneration of intervertebral disc
    Article Snippet: Equal amounts of total protein (20 μg) of each sample were separated on 5%–12% gradient SDS‐PAGE gel (P0012AC, Beyotime) and transferred to a 0.45‐um PVDF membrane (Merck, Germany). .. After the membrane was blocked by QuickBlock Western blocking solution (P0252, Beyotime, China) for 1 h, and subsequently incubated with different kinds of primary antibodies (Table ) at 4°C overnight with rotation (the housekeeping proteins used in this study were GAPDH). ..

    Article Title: Gli1 depletion induces oxidative stress and apoptosis of nucleus pulposus cells via Fos in intervertebral disc degeneration.
    Article Snippet: The protein samples were separated using SDS-PAGE and transferred to a 0.45-um PVDF membrane (Merck, Germany). .. Membranes were blocked by QuickBlock Western blocking solution (P0252, Beyotime, China) for 1 h and incubated with different kinds of primary antibodies (Table 1) at 4 C overnight with rotation (the housekeeping proteins used in this study were GAPDH (whole protein/cytoplasmic protein) and Lamin B1 (nuclear protein)). ..

    Article Title: Gli1 depletion induces oxidative stress and apoptosis of nucleus pulposus cells via Fos in intervertebral disc degeneration
    Article Snippet: The protein samples were separated using SDS-PAGE and transferred to a 0.45-um PVDF membrane (Merck, Germany). .. Membranes were blocked by QuickBlock Western blocking solution (P0252, Beyotime, China) for 1 h and incubated with different kinds of primary antibodies ( ) at 4 °C overnight with rotation (the housekeeping proteins used in this study were GAPDH (whole protein/cytoplasmic protein) and Lamin B1 (nuclear protein)). ..

    Membrane:

    Article Title: Dynamics of N6 ‐methyladenosine modification during aging and their potential roles in the degeneration of intervertebral disc
    Article Snippet: Equal amounts of total protein (20 μg) of each sample were separated on 5%–12% gradient SDS‐PAGE gel (P0012AC, Beyotime) and transferred to a 0.45‐um PVDF membrane (Merck, Germany). .. After the membrane was blocked by QuickBlock Western blocking solution (P0252, Beyotime, China) for 1 h, and subsequently incubated with different kinds of primary antibodies (Table ) at 4°C overnight with rotation (the housekeeping proteins used in this study were GAPDH). ..

    other:

    Article Title: Role of LECT2 in exacerbating atopic dermatitis: insight from in vivo and in vitro models via NF-κB signaling pathway
    Article Snippet: The PVDF membrane was blocked using the QuickBlockTM Western Blocking Solution (P0252, Beyotime, Shanghai, China) for 15 minutes.



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